Description
This assay employs a two-site sandwich ELISA to quantitate FAP in samples. An antibody specific for FAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FAP bound in the initial step. The color development is stopped and the intensity of the color is measured